T and B lymphocytes in non-Hodgkin's lymphoma.A comparison of tumor-derived cells and peripheral blood lymphocytes

Abstract. This study covered a population of 91 cows aged 3–6 years. Enzootic bovine leukaemia (EBL) was diagnosed with ELISA and PCR tests. The assays were performed in the 1st, 2nd, and 3rd month after calving. Use was made of anti-bovine monoclonal antibodies (Mab), (VMDR Inc. Pullman-USA) and conjugates of FITC and R-PE (Medac – Germany) with an indirect immunofluorescence reaction (IMF). A differentiation observed between the population numbers of CD19 + B lymphocytes and CD2 + T lymphocytes as well as the CD8 + T lymphocyte sub-population was found to be significant between cows with different genotypes of AcP. Moreover, a correlation was found between the polymorphism of AcP and EBL incidence in cows with reference to the number and percentage of CD19 + B lymphocytes (AcP polymorphism x EBL interaction). A significant differentiation in the profile of peripheral blood lymphocytes was observed between EBL-positive and EBL-negative cows as well as over the first three months after calving. The results reported in this study seem to indicate the potential contribution of a genetic predisposition connected with the expression of biological functions of the blood leukocyte acid phosphatase system in the activation and proliferation of these cells.

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CD200 and CD200R Expression on Peripheral Blood Lymphocytes and Serum CD200 Concentration as a New Marker of Endometriosis

Journal of Clinical Medicine ◽

10.3390/jcm9093035 ◽

2020 ◽

Vol 9 (9) ◽

pp. 3035

Author(s):

Monika Abramiuk ◽

Ewelina Grywalska ◽

Izabela Korona-Głowniak ◽

Paulina Niedźwiedzka-Rystwej ◽

Grzegorz Polak ◽

...

Keyword(s):

B Lymphocytes ◽

Disease Severity ◽

Peripheral Blood ◽

Cross Sectional Study ◽

Peripheral Blood Lymphocytes ◽

T And B Lymphocytes ◽

Cross Sectional ◽

Aberrant Expression ◽

Self Tolerance ◽

Cluster Of Differentiation

The causes of endometriosis (EMS) remain unknown; however, a number of immunological abnormalities contribute to the pathogenesis of the disease. The cluster of differentiation-200 (CD200) and its receptor (CD200R) maintain peripheral self-tolerance by negatively regulating immune responses. In this comparative cross-sectional study, we investigated the expression of CD200 and CD200R on T and B lymphocytes and the serum level of soluble CD200 (sCD200) using flow cytometry and ELISA, respectively. Peripheral blood samples were collected from 54 female patients and 20 healthy, age-matched controls. Results were tested for correlation with disease severity and selected clinical parameters. We demonstrated that the differences in sCD200 levels (p = 0.001), the frequencies of CD200-positive T and B lymphocytes (p < 0.001 and p = 0.004, respectively), and the frequencies of CD200R-positive T and B lymphocytes (p < 0.001 for all comparisons) in the study group correlated positively with disease severity. Receiver operating characteristic (ROC) analysis indicated that aberrant expression of CD200/CD200R might serve as a marker to distinguish between EMS cases. Finally, negative co-stimulatory factors may contribute to the induction and persistence of inflammation associated with EMS. It seems that it is essential to determine whether alteration in the CD200/CD200R pathway can be therapeutically targeted in EMS.

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Interference with oxidative processes inhibits proliferation of human peripheral blood lymphocytes and murine B-lymphocytes

International Journal of Immunopharmacology ◽

10.1016/0192-0561(91)90056-d ◽

1991 ◽

Vol 13 (7) ◽

pp. 1019-1026 ◽

Cited By ~ 20

Author(s):

Nicholas H. Hunt ◽

Elaine P. Cook ◽

Jean-Charles Fragonas

Keyword(s):

B Lymphocytes ◽

Peripheral Blood ◽

Human Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Blood Lymphocytes ◽

Human Peripheral Blood Lymphocytes ◽

Oxidative Processes

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Extracellular idiotypic immunoglobulin arising from human leukemic B lymphocytes.

Journal of Experimental Medicine ◽

10.1084/jem.152.6.1484 ◽

1980 ◽

Vol 152 (6) ◽

pp. 1484-1496 ◽

Cited By ~ 50

Author(s):

F K Stevenson ◽

T J Hamblin ◽

G T Stevenson ◽

A L Tutt

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Cell Surface ◽

B Lymphocytes ◽

Peripheral Blood ◽

Culture Fluid ◽

Peripheral Blood Lymphocytes ◽

Lymphocytic Leukemia ◽

Total Serum ◽

Blood Lymphocytes ◽

Idiotypic Antibody

The peripheral blood lymphocytes of nine out of nine patients with typical surface Ig-positive chronic lymphocytic leukemia but no paraprotein visible on serum electrophoresis have been shown by radioimmunoassay to export small amounts of pentameric IgM during culture (in the range of 2.4-7.2 ng/10(7) cells per h); three out of nine also exported monomeric IgD (0.7-1.4 ng/10(7) cells per h). Immunoglobulin turned over on the cell surface did not appear to contribute to material in the culture fluid, except possibly as vesicle-bound Ig. In three cases, which included two of the IgD producers, anti-idiotypic antibody raised against the cell surface Fab mu was used to demonstrate the idiotypic nature of the exported Ig. Anti-idiotypic antibody was also used to measure levels of idiotypic Ig in the sera of these three patients as a proportion of the total Ig. Total serum IgM was depressed in all three patients, and the idiotypic IgM represented 43%, 65%, and 96% of the IgM. The findings suggest that in typical chronic lymphocytic leukemia involving B lymphocytes, the export of a small amount of idiotypic Ig by the neoplastic cells in a common or even usual occurrence.

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Features of the immune disbalance during various clinico-pathogenetic variants of acute progressive pulmonary tuberculosis

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2010-3-42-50 ◽

2010 ◽

Vol 9 (3) ◽

pp. 42-50

Author(s):

O. V. Voronkova ◽

O. I. Urazova ◽

V. V. Novitsky ◽

Ye. G. Churina ◽

R. R. Khasanova ◽

...

Keyword(s):

Pulmonary Tuberculosis ◽

B Lymphocytes ◽

Peripheral Blood ◽

Functional Activity ◽

Proliferative Activity ◽

Peripheral Blood Lymphocytes ◽

Drug Resistant ◽

Cellular Composition ◽

Clinical Form ◽

Blood Lymphocytes

In article the data, concerning features of cellular composition, proliferative activity and cytokines production of peripheral blood lymphocytes is presented in patients with various clinico-pathogenetic variants of pulmonary tuberculosis: drag-sensitive and drug-resistant infiltrative, disseminated, fibrous-cavernous. It is established that the extensive destructive tuberculosis without dependence from the clinical form of disease is accompanied by the expressed insufficiency of the cellular-mediated mechanisms of immunological resistance both to drag-sensitive, and to drug-resistant M. tuberculosis against quantitative prevalence of B-lymphocytes and intensity of their functional activity.

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Celiac Sprue and Abdominal Lymphoma: Studies on the Cell-Mediated Immune Response of Peripheral Blood Lymphocytes

Canadian Journal of Gastroenterology ◽

10.1155/1988/838210 ◽

1988 ◽

Vol 2 (1) ◽

pp. 12-14

Author(s):

P. Davis ◽

H.J. Freeman ◽

A.B.R. Thomson

Keyword(s):

T Cells ◽

B Lymphocytes ◽

Peripheral Blood ◽

Cell Activity ◽

Peripheral Blood Lymphocytes ◽

Blood Lymphocytes ◽

Cell Mediated Immune Response ◽

Gluten Sensitive Enteropathy ◽

And Function ◽

Lymphocyte Responses

The development of primary abdominal lymphoma is a recognized complication of gluten-sensitive enteropathy (GSE). ln five patients with GSE plus lymphoma, the distribution and function of peripheral blood lymphocytes were determined and compared with 13 patients with GSE without lymphoma and with 28 normal control subjects. The percentage of T cells was lower in patients with GSE and GSE with lymphoma than in controls, whereas patients with GSE plus lymphoma had a significantly increased number of peripheral blood B lymphocytes when compared with GSE patients or controls. There was no difference in K cell activity or lymphocyte responses to mitogens and antigens between controls, GSE or GSE plus lymphoma patients. Prospective studies are needed in patients with GSE co investigate whether this fall in peripheral blood T cells and rise in B lymphocytes is a marker of concurrent lymphoma.

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